directed evolution

the scientist explained the directed evolution phase.

科学家解释了定向进化阶段。

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dr. lee uses directed evolution and protein engineering to improve enzymes.

李博士使用定向进化和蛋白质工程来改进酶。

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many research groups successfully rely on whole-gene random mutagenesis and recombination approaches for the directed evolution of enzymes.

许多研究小组利用整基因随机突变形成和重组方法，已成功的进行了酶的定向进化。

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dr. chen explained directed enzyme evolution in the lab.

陈博士在实验室里解释了定向酶进化。

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dr. lee gave a talk on from directed evolution to synthetic biology.

李博士做了一场关于从定向进化到合成生物学的报告。

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Then it would be autonomous evolution instead of directed evolution.

之后,取代定向进化的就将是自发的进化.

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the evolved enzyme with higher activity and higher regioselectivity is a suitable parent for further directed evolution to improve catalytic rates and enhance regioselectivity.

具有高活力、高区域选择性的突变酶的获得为进一步定向进化P450BM-3的催化性能提供了更好的进化模板。

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construction and screening of the directed evolution library of interferon-α family genes

人α型干扰素家族定向进化文库的构建及细胞筛选

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increasing activity of lipase from bacillus subtilis by directed evolution

定向进化提高枯草芽孢杆菌脂肪酶的活力

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Nowadays the application of mutator strain is a in vivo method in molecular biology for directed evolution of proteins.

目前致突变菌株（mutator strain）的使用是蛋白质定向进化中所采用的一种体内分子生物学方法。

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the recent researches indicated that the combined directed evolution, coupling the directed evolution with rational design and computational design approaches respectively, is becoming increasingly important in protein engineering and is becoming the new direction for directed evolution.

最近研究表明：定向进化和理性设计相结合、定向进化和以结构为基础的计算设计方法相结合正成为蛋白质工程中两个新的发展方向。

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a preliminary study on the directed evolution of d-hydantoinase by error-prone pcr

易错PCR法定向进化D-海因酶的初步研究

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in the past few years, many new protocols for directed evolution were developed on the basis of oligonucleotide-directed random mutation, error-prone pcr and dna shuffling.

最近几年，在过去常用的寡核苷酸介导的随机突变、易错PCR和DNA改组等方法的基础上又出现了一些新的定向进化方法。

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phage display and dna shuffling are important and efficient techniques for directed molecular evolution.

DNA重排和噬菌体展示及筛选是体外定向分子进化的重要核心技术。

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dr. lee conducted a directed evolution experiment in the lab.

李博士在实验室里进行了一项定向进化实验。

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studies on the directed evolution and thermostability of β-glucanase

β-葡聚糖酶的定向进化及热稳定性研究

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dr. lee uses a directed evolution technology platform for research.

李博士使用一个定向进化技术平台进行研究。

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scientists use directed evolution to improve enzymes.

科学家们使用定向进化来改良酶。

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scientists used directed evolution to improve the enzyme.

科学家们使用定向进化来改进这种酶。

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dr. smith studies in vitro directed enzyme evolution.

史密斯博士研究体外定向酶进化。

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the idea of directed molecular evolution is the extension and use of darwinian theory at molecular level.

定向分子进化的实质是达尔文进化论思想在分子水平上的延伸和应用。

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The successful display of hirudin on the surface of M13 phage laid a sound foundation for the further study on directed evolution of antithrombotic proteins with altered properties.

水蛭素在噬菌体表面的功成展示为进一步开展其实验定向进化以及结构与功能关系的研究打下基础。

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scientists use enzyme molecule directed evolution to improve catalysts.

科学家们使用酶分子定向进化来改进催化剂。

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the technology of directed evolution provides powerful tools for the development of novel biocatalyst without any structural or mechanistic information about the enzyme or about the reactions it performs.

定向进化技术已成为开发新型生物催化剂的有力工具，特别是在对酶结构或催化机理信息缺乏的情况下。

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scientists use in vitro molecular directed evolution to improve enzymes.

科学家们使用体外分子定向进化来改进酶。

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toward directed evolution of single-chain antibodies against human bladder carcinoma using synthetic phage display library

应用全合成噬菌体抗体展示库技术实现抗膀胱癌单链抗体的定向进化

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over the last three decades, directed evolution has emerged as a powerful technology platform in protein engineering.

在过去的几十年中，定向进化作为一种强大的技术平台强有力的托起了蛋白质改造这一伟大工程。

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recent advances in enzyme engineering have used a combination of these random methods of directed evolution with elements of rational enzyme modification to successfully by-pass certain limitations of both directed evolution and rational design.

酶工程的最新进展就是使这些定向进化的方法相结合，并与理性酶修饰方法的要素随机结合，通过各种组合选择出适合的方法来避免定向进化和理性设计的限制。

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in this article, the research topics of engineering proteins for thermostability, including rational design, directed evolution and consensus sequence method, are compared with each other.

比较了蛋白质耐热工程重点研究的合理化设计、定向改造和一致序列法等方法，并提出了将来的研究趋势。

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dr. lee used molecular directed evolution to improve the enzyme.

李博士使用分子定向进化来改进这种酶。

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