Application of Pollen Tube Pass Method to Transgenic Technology of Cotton

Pollen-tube Pathway Method and Its Application in Tobacco Breeding

花粉管通道法及其在烟草育种中的应用综述

互联网摘选

Development of Transgenic Wheat with Galanthus navies Agglutinin Gene ( s gna) via the Pollen_tube Pathway

利用花粉管通道法获得转雪花莲凝集素基因（sgna）小麦

互联网摘选

The pollen-tube pathway transformation technique can bypass the usual tissue culture and simplify soybean transformation procedures, but it is restricted in transgenic soybean because no sufficient evidence such as Southern-blot and heredity data to support exogenic gene to integrate into chromosome of soybean.

花粉管通道法转化大豆避免了组织培养过程，操作简便经济，但由于缺少Southern blot及后代遗传学分析证明外源基因整合进植物染色体上，在大豆转基因育种方面受到了限制。

互联网摘选

Transgenic rice plant was obtained by transferring the herbicide resistant gene bar into rice line "E32" by pollen-tube pathway method.

利用花粉管通道法将抗Basta除草剂的bar基因导入水稻品系E32，获得转基因植株。

互联网摘选

After 15 years studied, it is indicating that the gene from sorghum not only integrated into wheat but also expressed by introduced exotic DNA from sorghum ( 6A/ 115) into normal wheat through the pollen tube pathway.

经过我们15年的悉心研究，通过花粉管通道法将高粱DNA导入小麦，结果来自高粱的外源基因不但整合到小麦染色体，而且得到了表达。

互联网摘选

Two groups of rice variant lines increased their protein contents were obtained by introducing exogenous DNA from soybean into rice with soaking seeds, moreover irrigating seedlings, and through the pollen tube pathway after self pollination.

利用花粉管通道法和浸种及苗期浇灌法，将供体大豆DNA直接导入受体水稻，获得两种水稻变异株系。

互联网摘选

DNA transfer was studied using pollen tube passage method with wild rice ( Zizania) as donor and rice ( O. Sativa) as recipient.

本试验选用菰（Zizania）作供体，栽培水稻品种为受体，采用花粉管通道法进行DNA转移的研究。

互联网摘选

Exogenous total DNA extracted and purified from the donor ( Orychophragmus violaceas) was injected into the ovary of B. napus ( 3529-5) through the pollen tube channel after 24 hr artificial pollination.

以甘蓝型油菜（3529&5）为受体，诸葛菜为供体，用花粉管通道法，在自花受粉24小时后，导入外源总DNA。

互联网摘选

Research on Expression of HMW-GS1B × 14 Gene in Transgenic Wheat Via Pia Pollen Tube Pathway

高分子量麦谷蛋白亚基1B×14在花粉管通道法转基因小麦中的表达研究

互联网摘选

Studies on Introduction of Leaf Senescence-inhibition Gene P_ ( SAG12)-IPT into Common Wheat through Pollen-tube Pathway

利用花粉管通道法将叶片衰老抑制基因P（SAG12）-IPT导入普通小麦的研究

互联网摘选

The pollen tube pathway observation showed that the suitable time for introducing exogenous DNA was 18 hours to 48 hours after pollination.

授粉后72h，早期胚形成；④应用花粉管通道法对棉花导入外源DNA的适宜时期为授粉后18~48h。

互联网摘选

Via pollen-tube pathway the genomic DNA of the open-bud cotton Yi 9-3 was introduced into the insect-resistant cotton 707-1. The variant line with open-bud was obtained from their segregation generation.

本研究以异9&3柱头外露棉为供体，将提取的基因组DNA通过花粉管通道法经微量注射导入受体抗虫棉707-1的胚囊内，在分离的群体中的田间筛选到柱头外露的转化植株。

互联网摘选

Transgenic rice plants were obtained by transferring the arrowhead proteinase inhibitor gene ( API) into rice line 9311 through the pollen-tube pathway method.

采用花粉管通道法遗传转化技术，将慈姑蛋白酶抑制剂基因（API）导入水稻品系9311中，获得了转基因植株。

互联网摘选

With the pollen-tube pathway, the exogenous DNA that had been treated with radiation was introduced into Hashi 5 after its pollination and its descendants were selected and researched unceasingly.

采用花粉管通道法，在受体哈师5号授粉后，将经过辐照处理的供体DNA整体导入到哈师5号中，并对所得后代不断进行筛选和研究。

互联网摘选

The DNA of Glycine soja was introduced into wheat through the pollen tube pathway after self pollination. The T 2 generation population's agronomic traits have greatly changed.

用花粉管通道法将野生大豆总DNA导入小麦，获得了转基因小麦，其后代的农艺性状有较大的变化。

互联网摘选

Studies on culture of insect-resistant rice by transforming rice with PIN ⅱ gene via the pollen-tube pathway

PINⅡ基因通过花粉管通道法转化水稻的研究

互联网摘选

Via pollen-tube pathway the indica rice HD-357 was transformed with plasmid pTW-α containing Bar gene and PIN ⅱ gene.

利用花粉管通道法，用含抗除草剂基因bar及抗虫基因pin2的质粒pTW-α转化中籼HD357。

互联网摘选

Transformation of Rice Waxy Gene Via Pollen-tube Pathway

花粉管通道法转水稻Waxy基因

互联网摘选

Transgenic Rice Plant Resulting from Introduction of Arrowhead Proteinase Inhibitor Gene ( API) into Rice by the Pollen-tube Pathway Method

用花粉管通道法将慈姑蛋白酶抑制剂基因（API）导入水稻获得转基因植株

互联网摘选

Transgenic Rice Plant Obtained from Introduction of Herbicide Resistant Gene bar into Rice by Pollen-tube Pathway Method

用花粉管通道法将bar基因导入水稻获得可遗传的转基因植株

互联网摘选