patch clamp

Because the weakness of the signal, the background noise always dominates in the recordings, the threshold detector traditionally used in patch clamp to denoise and restore the channel signal can't work satisfactorily.

由于信号的微弱性，记录中噪声往往占明显优势，传统上采用阈值检测器来恢复通道信号，这需要人为的设定阈值，受主观影响很大，尤其是信噪比低时，阈值检测器失效。

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Record the influence of BK in single smooth muscle cell by patch clamp amplifer.

分离平滑肌细胞用膜片钳记录钙通道电流，观察BK对单个平滑肌细胞的影响。

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METHOD Using whole cell patch clamp technique.

方法应用全细胞膜片钳制技术.

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Methods After stimulating auditory thalamic radiation, excitatory postsynaptic currents ( EPSCs) were recorded at MGB neurons using whole-cell patch clamp technique in AC-MGB slices.

方法刺激听辐射纤维，采用脑片膜片钳全细胞技术在MGB分离和记录兴奋性突触后电流（EPSCs）；

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The widely using of patch clamp technique has greatly promoted our knowledge of ion channels.

膜片钳技术的广泛应用极大推动了人们对离子通道的认识.

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Methods: The whole cell recording patch clamp technique was used to investigate the Iks tail current density of enzymatic isolated M cell, sub-endocardial and sub-epicardial myocytes.

方法：利用全细胞膜片钳方法分析急性分离的M细胞和心内、外膜细胞的Iks通道尾电流密度。

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Methods Patch clamp technique was used to record sodium cur-rent in human atrial myocyte before and after administration of intracellular AA.

方法膜片钳全细胞记录技术记录应用花生四烯酸前后人心房肌细胞钠电流。

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Methods: Using inside out configuration of patch clamp single channel recording technique.

方法：采用内面向外膜片钳单通道记录技术。

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The patch clamp technique has become the main method for the molecule and cell researching.

膜片钳技术已成为分子和细胞学研究的重要手段.

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Whole-cell patch clamping: Whole cell currents were recorded in freshly isolated rabbit mesentery artery smooth muscle cells via the whole-cell patch clamp technique.

全细胞膜片钳（whole-cell patch clamp）技术：胶原酶法新鲜分离单个家兔肠系膜阻力血管平滑肌细胞，全细胞电压钳制状态下监测应用目的药物Oxy前后外向钾电流&钙敏感钾通道的电流变化。

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Abstract: The study of lens cells by patch clamp technique began in th e early 90 s.

文摘: 应用膜片钳技术对晶体细胞的研究始于90年代初.

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Methods: Whole-cell patch clamp technique was used to determine sodium channel cur rent.

方法: 采用全细胞膜片钳技术,记录钠通道电流.

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Patch-clamp technique was used to observe the changes of sodium channels caused by high concentration glucose.

利用膜片钳技术观察高糖条件下Mü ller细胞Na+通道的变化.

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Methods: Use in situ hybridization and patch clamp techniques.

方法: 采用原位杂交和膜片钳观察NMDA受体的数量和功能.

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Besides, using whole-cell patch clamp, we made preliminary investigation on the ASICs in the hypothalamus, the region of the brain that controls a large number of bodily functions.

此外，我们还运用全细胞膜片钳技术初步研究了下丘脑中的ASICs。

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A In-Pipette Pneumatic Micro-pressure Control System for Giga-Ohm Seal of Patch Clamp

膜片钳实验中吉欧封接的电极内气动微压力控制系统

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The patch clamp technique has become the main method for cell electrophysiology research.

膜片钳技术已成为细胞电生理学研究的重要手段.

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The study of lens cells by patch clamp technique began in the early 90 s.

应用膜片钳技术对晶体细胞的研究始于90年代初.

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To probe the effect of stress hormone corticosterone on the hippocampal cell membrane potential, the resting potentials of rat hippocampal cells in primary culture were measured with the patch clamp whole cell recording.

为探讨应激激素&皮质酮对海马神经细胞膜电位的影响，采用膜片钳全细胞记录的方法，测量了原代培养的大鼠海马神经细胞膜的静息电位。

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With patch clamp technic ( whole cell recording), the effect of dl-praeruptorin A ( Pra), an ingredient of Peucedanum praeruptorum Dunn on calcium current ( Ica) in the single ventricular cells of guinea pig was studied.

利用膜片钳制技术全细胞记录观察了白花前胡根中成分甲素对豚鼠单一心室肌细胞I（Ca）的作用。

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Methods The whole-cell patch clamp with current clamp was used.

方法采用全细胞膜片钳技术，进行电流钳制。

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Blind patch clamp study on auditory corticothalamic excitatory synaptic transmission

听觉皮质-丘脑兴奋性突触传递的盲法膜片钳研究

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METHODS: Single cell Ca 2+ imaging techniques and patch clamp technique were used to test the current of ion channel and action potential of Annexin A7 knock out mice embryonic heart cells.

方法：利用钙成像技术和膜片钳技术检测敲除Annexin a7小鼠的早期胚胎心肌细胞的钙平衡以及相应的离子通道的特性。

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Perforated patch clamp technique and its applications in channel current study

穿孔膜片钳技术在离子通道电流研究中的应用

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The effects of botanical alkaloids pesticide matrine on inward sodium channel gating properties of the central neurons isolated from the thoracic and abdominal ganglia of the cotton bollworm, Helicoverpa armigera ( H ü bner) were studied using whole-cell patch clamp technique.

用全细胞膜片钳技术研究了生物碱类植物杀虫剂苦参碱对棉铃虫Helicoverpa armigera幼虫离体培养中枢神经细胞钠离子通道门控过程的影响。

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Patch clamp recording revealed that the quality of root protoplasts was supe-rior with nutritive soil culture.

在膜片钳记录中对不同来源的根原生质体状态进行了比较。结果表明，土培养法分离的原生质体最适于膜片钳记录。

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Moreover, methods of the electronical signals recording of taste cell mainly depends on the microelectrode or the patch clamp sticking to the membrane of the cells.

不仅如此，目前的味觉电生理记录，仍主要依赖于以膜片钳为代表的细胞膜微电极穿刺或钳制技术。

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Methods Whole cell patch clamp recordings were made from RGCs in the acute retinal slices of Long Evans and Wistar rats aged postnatal 0-31 d. RGCs were stained with Lucifer Yellow diffused into cells during whole-cell recordings for the purpose of morphological study.

方法取出生后0～31d Long Evans和Wistar大鼠，各按睁眼时间分为两组，制备视网膜片，行膜片钳全细胞记录，同时行组织切片和细胞内染色，观察形态特征。

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With patch clamp method, the effects of QT on slow inward calcium current ( Ica) were investigated in single ventricular cells of guinea pigs.

应用全细胞膜片钳制技术观察了QT村豚鼠单一心室肌细胞钙离子电流（Ica）的影响。结果显示：QT能明显增加心室肌细胞的Ca2+内流，使Ica增大。

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Method Ion currents of rabbit ventricular myocyte were recorded using the whole cell patch clamp technique.

方法应用全细胞膜片钳技术记录兔心室肌单个细胞的电流.

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