Objective To construct plant expressing murine rotavirus antigen gene and transform it into Agrobacterium tumefaciens.

1、

Optimization of Agrobacterium-mediated Transformation Using the Immature Embryos and Introduction of Genes Coding HMW-GS Derived from Alien Species in Common Wheat

小麦幼胚农杆菌转化体系优化和HMW-GS基因转化研究

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2、

Construction of plant expression plasmid of fatty-acid alcohol oxidase gene from candida cloacae and its transformation into agrobacterium tumefaciens

Candida Cloacae源脂肪酸醇氧化酶基因的植物表达质粒构建及农杆菌转化研究

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3、

Scientists have used Agrobacterium-mediated genetic transformation to generate transgenic plants, and this method depends on the Ti plasmid.

在过去的几十年间，研究人员用农杆菌转化法获得了多种转基因植物，该转化法依赖于农杆菌中存在的Ti质粒。

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4、

The Study of Genetically Modified Oilseed Rape by Agrobacterium-About Bt and Cecropin

农杆菌转化Bt和抗菌肽融合基因获得转基因油菜的研究

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5、

Many useful transgenic plants identified by PCR and Dot-blotting were obtained by using this protocol.

应用此农杆菌转化系统，获得了一批经PCR和点杂交鉴定的转基因植株。

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6、

Wheat Agrobacterium-mediated Transformation with Different Variety Explants

小麦不同品种外植体的农杆菌转化方法的研究

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7、

An Agrobacterium-Mediated Transformation System Applicable for Four Genotypes of Maize

适用于4种玉米基因型的农杆菌转化方法的探讨

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8、

Study on the improving of wheat quality by pollen tube pathway transgene

花粉管通道法转基因改良小麦品质的初步研究

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9、

Probing into Cytological Embryology Mechanism of Pollen-tube Pathway Transgenic Technology

花粉管通道法转基因技术的细胞胚胎学机理探讨

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10、

Studies on the Influence Factors of Wheat Transformation in Pollen-tube Pathway

花粉管通道法转化小麦影响因素的研究

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11、

Application of Pollen Tube Pass Method to Transgenic Technology of Cotton

花粉管通道法在棉花转基因上的应用

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12、

Pollen-tube Pathway Method and Its Application in Tobacco Breeding

花粉管通道法及其在烟草育种中的应用综述

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13、

Development of Transgenic Wheat with Galanthus navies Agglutinin Gene ( s gna) via the Pollen_tube Pathway

利用花粉管通道法获得转雪花莲凝集素基因（sgna）小麦

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14、

The pollen-tube pathway transformation technique can bypass the usual tissue culture and simplify soybean transformation procedures, but it is restricted in transgenic soybean because no sufficient evidence such as Southern-blot and heredity data to support exogenic gene to integrate into chromosome of soybean.

花粉管通道法转化大豆避免了组织培养过程，操作简便经济，但由于缺少Southern blot及后代遗传学分析证明外源基因整合进植物染色体上，在大豆转基因育种方面受到了限制。

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15、

Transgenic rice plant was obtained by transferring the herbicide resistant gene bar into rice line "E32" by pollen-tube pathway method.

利用花粉管通道法将抗Basta除草剂的bar基因导入水稻品系E32，获得转基因植株。

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16、

After 15 years studied, it is indicating that the gene from sorghum not only integrated into wheat but also expressed by introduced exotic DNA from sorghum ( 6A/ 115) into normal wheat through the pollen tube pathway.

经过我们15年的悉心研究，通过花粉管通道法将高粱DNA导入小麦，结果来自高粱的外源基因不但整合到小麦染色体，而且得到了表达。

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17、

Two groups of rice variant lines increased their protein contents were obtained by introducing exogenous DNA from soybean into rice with soaking seeds, moreover irrigating seedlings, and through the pollen tube pathway after self pollination.

利用花粉管通道法和浸种及苗期浇灌法，将供体大豆DNA直接导入受体水稻，获得两种水稻变异株系。

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18、

DNA transfer was studied using pollen tube passage method with wild rice ( Zizania) as donor and rice ( O. Sativa) as recipient.

本试验选用菰（Zizania）作供体，栽培水稻品种为受体，采用花粉管通道法进行DNA转移的研究。

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19、

Exogenous total DNA extracted and purified from the donor ( Orychophragmus violaceas) was injected into the ovary of B. napus ( 3529-5) through the pollen tube channel after 24 hr artificial pollination.

以甘蓝型油菜（3529&5）为受体，诸葛菜为供体，用花粉管通道法，在自花受粉24小时后，导入外源总DNA。

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20、

Research on Expression of HMW-GS1B × 14 Gene in Transgenic Wheat Via Pia Pollen Tube Pathway

高分子量麦谷蛋白亚基1B×14在花粉管通道法转基因小麦中的表达研究

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